GCSF-PEG ELISA kit | EL-1611-73196-PEG-GCSF-PK
Cat. No: EL-1611-73196
$809.99 USD
| Reactivity | human |
|---|---|
| Detection range | 9.375ng/mL - 500 ng/mL |
| Sample type | Serum Plasma |
| Sample volume | 15ul |
| Assay time | 2.5 hours |
| Precision | < 25% for ULOQ and LLOQ and <20% for the remaining concentrations |
| Storage | -20C, 1 year |
The AffinityImmuno GCSF-PEG ELISA kit contains the components required for measuring natural and recombinant human GCSF-PEG in biological matrices such as human serum, plasma or cell culture supernatant.
Introduction
The AffinityImmuno GCSF-PEG (Pegylated Filgrastim) ELISA kit contains the components required for measuring natural and recombinant human GCSF-PEG in biological matrices such as human serum, plasma, or cell culture supernatant.
Principal of the Essay
This assay employs the indirect sandwich enzyme immunoassay technique. Anti -GCSF-PEG is coated onto 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. GCSF-PEG present in biological matrices is bound by the immobilized anti-GCSF-PEG antibody. After washing away any unbound substances, tagged anti-GCSF-PEG secondary antibody is added to the wells. After washing diluted detection reagent is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate is added to the wells for color development. The color development is proportional to the amount of GCSF-PEG present in the test samples. The color development is stopped and the intensity of the color is measured.